Fig. 2. Examination of perinatal inflammation in the absence of Nr4a1.

a Experimental design comparing wild type (wt) and Nr4a1 knockout (KO) dams exposed to perinatal neuroinflammation at E15.5. Two groups of animals were evaluated; one comparing only PBS vs. LPS and a second comparing PBS vs. LPS with subsequent treatments of MgSO₄/BMTZ. Embryo brains were harvested for molecular analysis, at 6 h following intrauterine injections of PBS or LPS. b–e Expression analysis for principal inflammatory genes Il1b, Il6, Tnf, and Tlr4 was examined in E15.5 brains. This analyses represents per condition N = 13–17 brains for group 1 and N = 7–12 brains for group 2. ^#p < 0.05, ^##p < 0.005, ^###p < 0.0005 by one-way ANOVA and *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005 by Tukey’s post-hoc test. Error bars represent ± SEM.