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. 2020 Jan 6;11(1):20. doi: 10.1038/s41419-019-2210-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b CD20 surface expression. DLBCL cell lines were treated with 5 µM Chidamide for 24 h and CD20 surface expression was determined by flow cytometry. Data shown are mean ± SD from three independent experiments. c CD20 mRNA expression. DLBCL cells were treated with 5 µM Chidamide for 24 h. Levels of CD20 mRNA expression were determined by RT-PCR. Numbers under each lane were ratios of CD20 mRNA to β-actin mRNA. d Effect of Chidamide on protein expression. DLBCL cells were treated with indicated dose of Chidamide for 24 h. The levels of HDAC1, HDAC2, HDAC3, p-HDAC3 (Ser424), HDAC10 and CD20 expression were determined by western blotting. Numbers under each lane were ratios of specific protein to GAPDH. e Effects of Chidamide and/or Rituximab on CD20 and ac-Histone-H3 (Lys27) protein expression. After treatment with 5 µM Chidamide and/or 10 µg/ml Rituximab, CD20 and ac-Histone-H3 (Lys27) expression was determined by western blotting. β-Actin was used as a loading control. Numbers under each lane were ratios of specific protein to β-actin. Data presented were from at least three independent experiments.