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. 2020 Jan 6;11(1):18. doi: 10.1038/s41419-019-2204-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Prediction of putative-binding sites between miR-578 and VEGF by miRDB. b Negative correlation between miR-578 expression and VEGF. c Luciferase reporter assay indicated that VEGF DEL abrogated the inhibition of VEGF promoter activity. **P < 0.01, vs control. d–g VEGF expression in cells expressing control, miR-578 or miR-578 inhibitor was detected by western blot and real-time PCR, respectively. Results represented the mean ± s.d. of three independent experiments. **P < 0.01, vs control. miR-578 in, miR-578 inhibitor.