Fig. 5. Human cells lacking BCL-XL show poor engraftment in xenograft mice.

a–d Lentivirally transduced or untransduced human HSPC were transplanted in Rag2^−/−γc^−/− mice after sub-lethal irradiation. Mice were sacrificed 7–8 weeks after transplantation and bone marrow (BM) and spleen were analyzed for the presence of various human hematopoietic populations by flow cytometry (a, b) as well as for GFP expression in the individual subpopulations (c, d). Bars represent mean ± SEM, n = 6-10 from six independent experiments. p-values: b **p = 0.0047 for control vs shBCL-XL CD33⁺ cells; **p = 0.003 for shLuci vs shBCL-XL CD33⁺ cells; *p = 0.035 control vs shBCL-XL CD19⁺IgM⁻ cells; *p = 0.0431 for control vs shLuci CD19⁺IgM⁺ cells; *p = 0.014 for control vs shBCL-XL CD19⁺IgM⁺ cells (c) **p = 0.0017, *p = 0.0312 (d) **p = 0.0017 for CD34⁺ and CD19⁻10⁺ cells; ***p = 0.001; **p = 0.003 for CD19⁺IgM⁻, and CD19⁺IgM⁺ cells.