Figure 3.

Transdiaphragmatic pressure (Pdi) generation across motor behaviors in 24 (n = 7), 27 (n = 7), and 30 (n = 8) month old female (F; closed circle) and male (M; open triangle) C57BL/6 × 129 mice: eupnea, hypoxia (10% O₂)‐hypercapnia (5% CO₂) and tracheal occlusion, presented as absolute values (a), and normalized to Pdi_max (b). Data points are the average Pdi amplitude from each animal. Boxplot represents median, 25th and 75th percentile and whiskers 10th and 90th percentiles. Data were analyzed with a mixed linear model with an animal as a random effect (age × behavior × animal). (a) Main effect of behavior (p < .01), no effect of age (p = .65), or interaction (p = .68). (b) Main effect of behavior (p < .01), no effect of age (p = .64), or interaction (p = .77) *significantly higher Pdi generation during tracheal occlusion compared to eupnea and hypoxia‐hypercapnia