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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Eukaryot Microbiol. 2019 Aug 8;67(1):28–44. doi: 10.1111/jeu.12751

Figure 9. Tomogram, segmentation and 3D models of a Polar Tube.

Figure 9.

A. Tomogram of a portion of PT containing cargo and membranes, and its surface is covered with tufts of fibrillar material. Segmentation overlay of a portion of PT tomogram (S-3). The different PT structures are color coded to the various densities visible in the stacks of images and identified by color. The tomogram was segmented and 3D models generated from it using Amira® software.

B. Amira generated 3D model of a portion of polar tube obtained from the tomogram images. The colors are assigned to different structures inside and on the surface of the polar tube. The fibril tufts are visible on the surface of the PT. Image was taken at the tilt angle of 0° degrees at Y direction.

C. Amira generated 3D model of a portion of polar tube obtained from the tomogram images. Image was taken at the tilt angle of −60° degrees at Y and Z direction.

D. Amira generated 3D model of a portion of polar tube obtained from the tomogram images. Image was taken at the tilt angle of −60° degrees at X and Y direction.