Table 2.
Bacteria | Primer sequence (5′–3′)1 | A T 2 (°C) | Size (bp) |
---|---|---|---|
Firmicutes | F: TGAAACTCAAAGGAATTGACG | 60 | 157 |
R: ACCATGCACCACCTGTC | |||
| |||
Bacteroidetes | F: GGAACATGTGGTTTAATTCGATGAT | 60 | 126 |
R: AGCTGACGACAACCATGCAG | |||
| |||
Roseburia | F: TACTGCATTGGAAACTGTCG | 60 | 230 |
R: CGGCACCGAAGAGCAAT | |||
| |||
Prevotella | F: CACCAAGGCGACGATCA | 60 | 283 |
R: GGATAACGCCTGGACCT | |||
| |||
Ruminococcus | F: GAGTGAAGTAGAGGTAAGCGGAATTC | 60 | 220 |
R: GCCGTACTCCCCAGGTGG | |||
| |||
Universal | F: ACTCCTACGGGAGGCAGCAGT | 60 | 177–179 |
R: GTATTACCGCGGCTGCTGGCAC |
1F: forward primer; R: reverse primer. 2AT: annealing temperature.