Table 2.
Sequences of primers and probes for intestinal bacteria.
| Bacteria | Primer sequence (5′–3′)1 | A T 2 (°C) | Size (bp) |
|---|---|---|---|
| Firmicutes | F: TGAAACTCAAAGGAATTGACG | 60 | 157 |
| R: ACCATGCACCACCTGTC | |||
|
| |||
| Bacteroidetes | F: GGAACATGTGGTTTAATTCGATGAT | 60 | 126 |
| R: AGCTGACGACAACCATGCAG | |||
|
| |||
| Roseburia | F: TACTGCATTGGAAACTGTCG | 60 | 230 |
| R: CGGCACCGAAGAGCAAT | |||
|
| |||
| Prevotella | F: CACCAAGGCGACGATCA | 60 | 283 |
| R: GGATAACGCCTGGACCT | |||
|
| |||
| Ruminococcus | F: GAGTGAAGTAGAGGTAAGCGGAATTC | 60 | 220 |
| R: GCCGTACTCCCCAGGTGG | |||
|
| |||
| Universal | F: ACTCCTACGGGAGGCAGCAGT | 60 | 177–179 |
| R: GTATTACCGCGGCTGCTGGCAC | |||
1F: forward primer; R: reverse primer. 2AT: annealing temperature.