Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The S837A mutant has enhanced levels of PDR5 transcript. Q-RT-PCR was performed as described in the Materials and Methods. The ΔCT values for the three references genes were determined and these values were used to obtain the ΔΔCT between the WT and mutant strains for each reference gene. The times difference was calculated from the formula: fold change = 2ΔΔCT. For the ALG9 and TAF10 experiments, n =4. For the TDH3 determination, n =3.
