Figure 12.
The S837A mutation increases the PDR5 transcript half-life. RNA was labeled continuously with 4-TU for three hrs. Following this, cells were chased with 5 mM uracil for various times before extracting the RNA and recovering the 4-TU-containing transcripts as described in the Materials and Methods. Following q-RT PCR, the CT values were used to calculate the % 4-TU remaining at 15, 30, and 45 min with the value at 0 min as 100%. Data were plotted using the one-phase exponential decay equation: y= span e-k.x + plateau where k is the rate constant, x is the time, y is the log % 4-tu remaining after the uracil chase. In all of these plots, ▪▪▪ = WT (JG2015, green line); ● = S837A (JG2175, orange line). In these experiments, n = 3.