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Preliminary testing of phosphoserine mutants indicated that they all increase drug resistance. Cultures of six alanine substitution mutants (JG2175-JG2181) were tested for resistance to clotrimazole, cycloheximide, cyproconazole, and cerulenin in liquid YPD cultures (48 h at 30 °C) at several concentrations as described in the Materials and Methods. In these experiments, n ≥ 4.
