Figure 8.

The ATPase activity is doubled in PM vesicles prepared from the S837A mutant. PM vesicles were prepared as described by Kolaczkowski et al. (1996). (A) The assay of ATPase activity was performed as previously described (Arya et al. 2019) with 3 mM ATP. Activity was measured in Tris-glycine buffer (pH 9.5). Activity was assayed in two different PM vesicle preparations prepared from the S837A mutant (JG2175) and three from the WT strain, JG2015. (B) ATPase activity was measured as a function of ATP concentration in PM vesicles prepared from WT (▪▪▪, green line) and S837A (●, orange line) strains in the conditions described in the Materials and Methods.