Table 2. Yeast strains used in this study.
| Strain designation | Genotype | Reference |
|---|---|---|
| R-1a | MATα his1, ura3, PDR1-3, pdr5::KanMX4, snq2, yor1, pdr3, pdr10, ycf1 | Sauna et al. 2008 Derived from AD1-7 |
| JG2001 | MATα his1, ura3, PDR1-3, PDR5, snq2, yor1, pdr3, pdr10, ycf1 The Δpdr5 gene in AD1-7 replaced by PDR5 | Golin et al. 2007 |
| JG2015 | Isogenic to R-1 but has the addition of pSS607containing wild-type PDR5 and URA3 genes | Sauna et al. 2008 |
| JG2015*b | Retransformation of R-1 with pSS607, but selection on 5-foa medium of a ura3, PDR5 strain (no longer contains KanMX4). | This study |
| JG2063 | Isogenic to R-1, but containing a G312A mutation | Furman et al. 2013 |
| JG2133 | Isogenic to R-1, but containing an A666G mutation | Arya et al. 2019 |
| JG2175 | Isogenic to R-1, but containing an S837A mutation | This study |
| JG2176 | Isogenic to R-1, but containing an S837D mutation | This study |
| JG2177 | Isogenic to R-1, but containing an S840A mutation | This study |
| JG2178 | Isogenic to R-1, but containing an S841A mutation | This study |
| JG2179 | Isogenic to R-1, but containing an S849A mutation | This study |
| JG2180 | Isogenic to R-1, but containing an S850A mutation | This study |
| JG2181 | Isogenic to R-1, but containing an S854A mutation | This study |
| JG2182 | Isogenic to R-1, but containing S837A, S854A mutation | This study |
| JG2183 | Isogenic to R-1, but containing S837A, S850A mutation | This study |
a
All site-directed mutations were made in pSS607 (Golin et al. 2007) and introduced into the R-1 strain by transformation. The desired ura3 derivatives were selected on 5-foa medium and verified as described previously (Ananthaswamy et al. 2010).
b
JG2015* is a recreation of JG2015 created specifically for this study.