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. 2019 Dec 2;21(1):e48741. doi: 10.15252/embr.201948741

Figure EV2. YAP S94A‐expressing cells are similar to vector control.

Figure EV2

  1. Overexpression of YAP or YAP S94A does not significantly alter the expression of endogenous AIB1, YAP, TAZ, or TEADs. Western blot analysis of indicated proteins following YAP S94A or YAP overexpression compared to parental baseline. Asterisk indicates non‐specific band.
  2. YAP S94A does not interact with TEAD. Co‐immunoprecipitation of myc‐tagged YAP S94A or YAP performed with myc antibody. Immunoblots show co‐IP and input lysate; membranes were probed with antibodies as indicated.
  3. Gene expression changes between YAP and YAP S94A are not significantly different from YAP relative to vector control (GSE70506) and are significantly correlated. Waterfall plot comparing significantly regulated genes (> 2FC an P < 0.05), to significantly regulated genes following removal of de novo YAP signature genes (Fig 1) in MCF10A YAP vs. vector control to YAP vs. YAP S94A.
  4. De novo YAP signature expression changes between YAP and vector control are not significantly different than YAP relative to YAP S94A. Volcano plot showing change of YAP‐activated and YAP‐repressed genes (described in Fig 1A and B) in YAP vs. vector control.
  5. Knockdown of AIB1 does not affect stabilization of YAP, TAZ, or TEADs. Western blot analysis of indicated proteins following AIB1 knockdown.

Source data are available online for this figure.