Figure 1. Expression, endoproteolysis, and APP processing of Aβ43‐generating PS1 FAD mutants.

1. Immunoblot analysis of presenilin expression and endoproteolysis in cell lysates of untransfected HEK293/sw cells endogenously expressing presenilin and single‐cell clones overexpressing PS1 WT or the indicated PS1 mutants.
2. Immunoblot analysis of total Aβ secretion and the production of different Aβ species (shown in low and high immunoblot exposures) in conditioned media of cell lines described in (A).
3. Mass spectrometry analysis of Aβ species immunoprecipitated from conditioned media of cell lines described in (A).

Source data are available online for this figure.