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. 2019 Nov 25;21(1):e47996. doi: 10.15252/embr.201947996

Figure EV5. RO7019009 maintains activity toward CHAPSO‐solubilized γ‐secretase.

Figure EV5

  1. Sandwich immunoassay of short Aβ species (Aβ37 + Aβ38) generation from recombinant APP C100‐His6 substrate by CHAPSO‐solubilized γ‐secretase of HEK293/sw cells overexpressing WT or mutant PS1 in the presence of RO7019009 or vehicle (DMSO) (n = 3 biological replicates).
  2. Immunoblot analysis of AICD generation from recombinant APP C100‐His6 substrate by CHAPSO‐solubilized γ‐secretase of HEK293/sw cells overexpressing WT or mutant PS1 in the presence of RO7019009, L‐685,458 (5 µM), or vehicle (DMSO).
  3. Quantitation of AICD generation in (B) (n = 4–5 biological replicates).
Data information: Data in (A) and (C) are presented as mean ± SD.Source data are available online for this figure.