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. 2019 Feb 1;12(2):419–430.

Figure 6.

Figure 6

miR-429 inhibits RhoA expression in LAC cells. A. WT-RhoA 3’UTR luciferase reporter vector, and a MUT-RhoA 3’UTR luciferase reporter vector with mutations on miR-429 binding sites of the RhoA 3’UTR was constructed; B. WT-RhoA/MUT-RhoA and miR-429-NC/miR-429-mimic were transfected into A549, and luciferase activity was detected; C, D. Western blotting was used to measure the protein expression of RhoA in PG49 and A549; E. Western blotting was used to measure the protein expression in WT-A549, RhoA knockout-A549 and A549 cells that miR-429-NC/miR-429-mimic were transfected into. Three independent repeats per experiment. Compared with the si-NC or miR-429-NC, ***was P<0.001. Compared with the WT group, #was P<0.05, ##was P<0.01 and ###was P<0.001.