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. 2019 Mar 1;12(3):843–850.

Figure 5.

Figure 5

Overexpression of miR-195 can downregulate MEK1 expression, inhibit bladder cancer cell proliferation, and induce apoptosis. A. qRT-PCR was used to detect the expression of miR-195 in bladder cancer cells. B. qRT-PCR was used to detect the expression of MEK1 mRNA in bladder cancer cells. C. Western blot was used to detect the expression of MEK1 protein in bladder cancer cells. D. qRT-PCR was used to detect miR-195 expression in bladder cancer cells of each transfection group. E. Western blot was used to detect the protein expression of bladder cancer cells in each transfection group. F. Flow cytometry was used to detect the apoptosis rate of bladder cancer cells in each transfection group. G. Flow cytometry was used to detect the proliferation ability of bladder cancer cells in each transfection group. *P<0.05, compared with SV-HUC-1 cells. #P<0.05, compared with miR-NC.