Figure 1.

Gut barrier dysfunction in Apc^Min/+ mice with colon cancer cachexia. A. Gut permeability was measured between the Apc^Min/+ mice and the WT mice at 8, 14, and 20 weeks of age. Serum fluorescein dextran concentrations were measured one hour after gavage (n=5). B. Mice were gavaged with 200 μl of Evans blue, and gut transit was determined by recording the time until Evans blue appeared in the feces (WT=7, Apc^Min/+=5). C. Cachexia (red arrow) in the intestine at 14 and 20 weeks of age in Apc^Min/+ mice. Intestinal inflammation (black arrow) in the intestine at 20 weeks of age in Apc^Min/+ mice. D. Lactescent serum of the Apc^Min/+ mouse was observed at 20 weeks of age. Data are expressed as the Mean ± SEM. The differences between the mean values were assessed by Student’s t-tests and analyzed using GraphPad Prism software 7.0. *P<0.05; ***P<0.001; ns, not significant.