Figure 6.

Bile acids affected intestinal cholesterol absorption in Apc^Min/+ mice. A. FXR and ASBT were measured by qRT-PCR in the intestines of Apc^Min/+ and WT mice (n=3). Data are expressed as the mean ± SEM. The differences between the mean values were assessed by Student’s t-tests and analyzed using GraphPad Prism software 7.0. **P<0.01. B. Bile acids may inhibit cholesterol uptake in the intestine of Apc^Min/+ mice. Cholesterol uptake is mediated by NPC1L1, CD36, and ABCG5/G8 in enterocytes. PPARα activation reduces cholesterol uptake and bile acids and may reduce FXR activation. FXR inhibits ASBT through reduced SHP activation. NPC1L1: Niemann-Pick C1 Like-1; ABCG5/G8: ATP-binding cassette transporter G5/G8; CD36: cluster of differentiation 36; PPARα: peroxisome proliferator-activated receptor α; FXR: farnesoid X receptor; ASBT: apical sodium-dependent bile acid transporter; SHP: short heterodimer partner.