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. 2019 Mar 1;12(3):717–729.

Figure 3.

Figure 3

The target gene of lncRNA TUG1 is miR-21. A. Sequence alignment between lncRNA TUG1 with miR-21 (left). Dual luciferase reporter assay was used to measure the relative fluorescence activity in NRK-52E cells co-transfected with NC-mimics and TUG1-wt, miR-21 mimics and TUG1-wt, NC-mimics and TUG1-mut, or miR-21 mimics and TUG1-mut. Student’s t-test: ***P < 0.001 (right). B. RNA binding protein immunoprecipitation (RIP) assay determined the interaction between lncRNA TUG1, miR-21 with AGO2, Student’s t-test: ***P < 0.001. C. qRT-PCR detected the expression level of miR-21 in db/db DN mice and HG-stimulated NRK-52E cells (top panel). qRT-PCR was also used to evaluate the mRNA expression levels of lncRNA TUG1 or miR-21 in cells transfected with pc-DNA, pc-TUG1, si-NC, and si-TUG1 respectively (bottom panel). Student’s t-test: ***P < 0.001.