Fig 7.

Interaction between HLA-DR⁺CD8⁺T cells and autologous neutrophils and monocytes (a) CD8⁺T from HV cultured for 3 days in the presence of 25% plasma derived from AD patients (n = 8) induces a similar phenotype to the ex vivo CD8⁺T cells from cirrhotic patients contrary to HV CD8⁺T cells cultured in the presence of 25% HV plasma (n = 10). (b) Phenotype of autologous fresh neutrophils following 24 hr co-culture with AD cirrhotic-plasma (HLA-DR^high) vs HV-plasma (HLA-DR^low) treated CD8⁺T cells. (c) Phagocytic ability of neutrophils following 4 h co-culture with HLA-DR^highvs HLA-DR^low CD8⁺T cells (left panel) as well as following the removal of HLA-DR^high from total CD8⁺T cell population (right panel). (d) Cytokine productions in neutrophils following 4 h of co-culture. (e) Phenotypic changes on co-cultured monocytes with plasma pre-conditioned CD8⁺T cells (HV-plasma (n = 4) and AD cirrhotic patients plasma (n = 7)) at 24 h. * p<0.05. (Wilcoxon signed-rank test (a;b;d;e), Paired t-test (c)).