Fig. S3.

Phenotypic and functional characteristics of HLA-DR+CD8+ T cells isolated from HV. (a) Perforin and Granzyme B levels in HLA-DR+CD8+ T cells from HV (n = 6) and patients (n = 9). (b) In vitro cytokines production of HLA-DR- or HLA-DR+ CD8+ T cells from HV following 48h CD3/CD28 beads stimulation (n = 8). (c) Expression of classical T-regulator cells markers in HLA-DR+CD8+ T cells and paired HLA-DR-CD8+ T cells from HV (n = 5). (d) Proliferation of autologous PBMCs following co-culture in the presence of HLA-DR+ or HLA-DR-CD8+ T cells from HV (n = 7) (1:2 HLA-DR+ or HLA-DR- CD8+ T cell to PBMCs ratio) (left panel). The effect of checkpoint receptor blockade was assessed in the presence of HLA-DR+CD8+ T cells (n = 3) (right panel). * p<0.05 * p<0.05; ** p<0.0001 (Mann-Whitney U test (a); Wilcoxon signed-rank test (b;c;d))