Fig. 2.

Characterization of 6-AN. (a) Chemical Structural formula and basic information. (b) HepAD38 and HepG2-NTCP cells were treated with the indicated concentrations of 6-AN for 72 h. Effects of 6-AN on the viability of HepAD38 and HepG2-NTCP cells were determined by the MTT assay. (c) The CC50 determined by the MTT assay in the indicated cell lines. (d) EC50 determined by the HBsAg ELISA. Working concentration of 6-AN were set from 200 μM to 0.39 μM in a descending order and 0 μM was set as control. HepAD38 cells were incubated with the medium containing different concentrations of 6-AN for 3days. (e) Effect of 6-AN on CMV promoter. Renilla luciferase reporter vector was transfected into HepG2 cells, which were treated with 12.5 μM and 25 μM 6-AN at 12 h post-transfection. The luciferase activity was measured at 48 h after 6-AN treatment. Results are expressed as the average of three independent experiments (n = 3 per group). The mean value ± standard error is indicated. (*P < 0.05; ^⁎⁎P < 0.01).