Fig. 5.

Overexpression of ADAM17 reverses the protective effects of macrophage HIF-1α deficiency on aortic dissection development.

(a-b) Schematic of the experimental procedures and representative pictures of aortas obtained from HIF-1α^fl/fl and HIF-1α^△lysm mice; Aortic dissection incidence among HIF-1α^fl/fl and HIF-1α^△lysm mice injected with or without AAV2 ADAM17 and fed with BAPN (0.5%) for 28 days (n = 12).

(c) Representative images of transverse cryosections of aortas after Verhoeff-Von Gieson staining, and the quantification of elastin degradation (n = 6). Scale bar, 100 µm.

(d) Immunohistochemical staining for MMP2 (red) and MMP9 (red) (n = 3). Scale bar, 100 µm.

(e) The concentration of various cytokines (IL-12, TNF-α, IFN-γ, MCP-1, IL-10, IL-6) in plasma of BAPN-treated HIF-1α^fl/fl and HIF-1α^△lysm mice with or without injection with AAV2 ADAM17 (n = 5).

The data are presented as the mean ± SEM. For the nonparametric tests, Kruskal-Wallis test was used for comparisons between three or more groups (c). Statistical significance is indicated by *P < 0.05, compared with HIF-1α^△lysm mice (c); One-way ANOVA with Tukey's post hoc test (e) was performed. Statistical significance is indicated by *P < 0.05, compared with HIF-1α^△lysm mice (e). Statistical significance is indicated by ^#P < 0.05, compared with HIF-1α^fl/fl mice (e).