Fig. 6.

Acriflavine treatment ameliorates aortic dissection formation via the inhibition of HIF-1α in macrophages.

(a-b) Schematic of the experimental procedures and representative pictures of aortas obtained from HIF-1α^fl/fl and HIF-1α^△lysm mice (n = 12); Aortic dissection incidence among HIF-1α^fl/fl and HIF-1α^△lysm mice injected with and without acriflavine (every 3 days) and fed with BAPN (0.5%) for 28 days (n = 12).

(c) Representative images of transverse cryosections of aortas after Verhoeff-Von Gieson staining, and the quantification of elastin degradation (n = 6). Scale bar, 100 µm.

(d) Schematic view of macrophage HIF-1α regulating proinflammatory response and disruption of elastic fibers via a HIF1α-ADAM17 pathway during aortic dissection development.

The data are presented as the mean ± SEM. For the nonparametric tests, Kruskal-Wallis test was used for comparisons between three or more groups (c). Statistical significance is indicated by *P < 0.05, compared with HIF-1α^fl/fl mice (c).