Fig. 3.

Arrayed library CRISPR screen. Arrayed libraries are generated in multi-well plates, where each well contains constructs preparation targeting an individual gene or genomic locus. Arrayed libraries are delivered to populations of cells grown in an arrayed format as well, preventing an individual cell from being transduced with multiple sgRNAs with different targets. There may be selection steps and treatments involved, but this can vary depending on the screen. Phenotypes are identified rather than necessarily being selected for (allowing for reverse genetic screening), since the sgRNA responsible for each phenotype is known based on well location in the original annotated library. The final outcome is a ranked phenotypic measure for each sgRNA delivered in the screen.