Table 1.
Genome-Scale CRISPR-mediated Chemogenetic Screens in Human Cells.
| Approach | Guides/gene | Genes | Cell Type | Phenotype [Reference] |
|---|---|---|---|---|
| CRISPRko | (Sabatini) 10 | 7114 | Near-haploid KBM7 and pseudo-diploid HL60 leukemia cells | Resistance to thioguanine and etoposide [37] |
| CRISPko | (GeCKO) 3-4 | 18,080 | A375 melanoma cells | Resistance to vemurafenib [38] |
| CRISPRko | (TKOv1) 6 | 17,661 | RPE1-hTERT, HeLa and SUM149PT cells | Sensitivity and resistance to olaparib [46] |
| CRISPRko | (TKOv3) 4 | 18,053 | 293A, HCT116 and MCF10A cells | Sensitivity and resistance to ATR inhibition [47] |
| CRISPRko | (TKOv1) 6/(TKOv3) 4 | 17,661 | HeLa, HCT116, RPE1 hTERT TP53−/− cell | Resistance to ATR inhibition [48] |
| CRISPRko | (custom) 5 | 18,080 | HCT116 cells | Sensitivity and resistance to NAMPT inhibitor [42] |
| CRISPRko | (custom) 5 | 18,080 | HCT116 cells | Sensitivity and resistance to cavinafungin [43] |
| CRIPSRi | 10 | 49 | K562 leukemia cells | Sensitivity to AB toxin ricin [43] |
| CRIPSRi | 10 | 15,977 | K562 leukemia cells | sensitivity to rigosertib [50] |
| CRIPSRa | 10 | 49 | K562 leukemia cells | sensitivity to AB toxin ricin [49] |
| CRISPRa | 10 | 15977 | K562 leukemia cells | Sensitivity to rigosertib [50] |
| CRISPRa | 3 | 23,430 | A375 melanoma cells | Sensitivity to BRAF inhibitor (PLX-4720) [51] |
| CRISPRi | (hCRISPRi-v2) 5 | 19,050 | A375 melanoma cells | Resistance and sensitivity to vemurafenib [52] |
| CRISPRa | (hCRISPRa-v2) 5 | 19,050 | A375 melanoma cells | Resistance and sensitivity to vemurafenib [52] |
| CRISPRko | (GeCKOv2) 6 | 19,050 | A375 melanoma cells | Resistance and sensitivity to vemurafenib [52] |
| CRISPRa | up to 12 | every coding and 4000 non-coding transcripts | K562 leukemia cells | Resistance and sensitivity to imatinib [55] |
| CRISPRko | total 18,315 | noncoding loci surrounding CUL3, NF1, NF2 | A375 melanoma cells | Resistance to vemurafenib [57] |
| CRISPRa | 10 | 10,504 lncRNA TSS | A375 melanoma cells | Resistance to vemurafenib [58] |
| CRISPRa | (Konermann et al. 2015) 3 | 23,430 coding isoforms | MOLM14 AML cells | Resistance to Ara-C [59] |
| CRISPRa | 4 | 14,701 lncRNA genes | MOLM14 AML cells | Resistance to Ara-C [59] |
| CRISPRko | (Brunello) 4 and (Gattinara) 2 | 19,114 | Meljuso and A375 melanoma cells, OVCAR8 ovarian cells | Resistance and sensitivity to A-1331852 [56] |
| CRISPRko | (Brunello) 4 and (Gattinara) 2 | 19,114 | Meljuso and A375 melanoma cells and OVCAR8 ovarian cells | Resistance and sensitivity to S63845 [56] |
| CRISPRko | (Brunello) 4 | 19,114 | OVCAR8 ovarian cells and A375 melanoma cells | Resistance and sensitivity to olaparib [56] |
| CRISPRko | (Brunello) 4 | 19,114 | Hap1 cells | Resistance and sensitivity to talazoparib [56] |
| CRISPRko | (Gattinara) 2 | 19,114 | A375 melanoma cells | Resistance and sensitivity to talazoparib [56] |