Figure 4.

Effect of simultaneous versus sequential injection of microparticles and collagenase on microparticle delivery in the SCS. A first injection (inj1) was made into the SCS of rabbit eyes ex vivo: (i) control solution containing microparticles in HBSS (Particle), (ii) collagenase formulation without microparticles (Colla CaCl₂) in HBSS, (iii) collagenase formulation with microparticles (Particle Colla CaCl₂) in HBSS and (iv) HBSS. After incubation at 37 ° C for 4 h, a second injection (inj2) was made into the SCS at the same injection site: (i) no injection (ii) control microparticle solution (Particle), (iii) no injection and (iv) control microparticle solution (Particle). Representative fluorescence micrographs (a), particle delivery coverage (b), particle distribution (c), and APD (d) of red-fluorescent microparticles after the second injection in the SCS. ^ǂAverage particle distance. Graphs show mean ± SEM (n=6 in (i and iii), n=3 in (ii), and n=5 in (iv)). *,*** indicate significant difference (one tailed t-test, p<0.05,0.005, respectively).