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. 2020 Jan 6;10:2. doi: 10.1186/s13578-019-0367-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 12 — Proliferation of hAMSCs isolated from human amniotic membrane by dual enzyme method during a 7-day culture period (i.e. cell expansion for the 2nd passage of cells) on the different types of microsphere (chitosan-based microspheres (CMs, GCMs and CCMs) and normal plate. Live plus dead hAMSCs isolated from the dual enzyme digestion were counted in a haemocytometer. Mean values ± SE (n = 30 microspheres of each type) represented by the bars were calculated using SPSS software, statistical significance is indicated (*p < 0.05; **p < 0.01)