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. 2020 Jan 1;17(1):125–136. doi: 10.7150/ijms.35693

Figure 1.

Figure 1

a) Autologous fibroblasts in the second passage. Cell division in a monolayer of fibroblasts (A); thin elastic fibers between cells (B); and collagen type I with a red-yellowish color and strong birefringence (C). Safranin O/Fast green; B. Weigert's method; C. Sirius Red. Objective magnification: x 40. b) Morphology of skin. Arrangement of type I collagen bundles in the papillary dermis and the reticular dermis (A) stained with H-E; localization of type III collagen (reticular fibers) after silver staining (B) and thin collagen bundles in the dermis with predominantly yellowish color (C). (A x 20; B x10, C x 10). c) Immunolocalization of fibrillin-1-rich microfibrils (yellow arrows) (A, B) and cytochrome P450 aromatase in fibroblasts -yellow arrows (C). (A x 20; B x 40. C x 40).