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. 2020 Jan 6;20:5. doi: 10.1186/s12935-019-1071-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Circ_LARP4 works as a sponge for miR-513b-5p to modulate LARP4. a Three websites (PITA, microT and miRmap) were utilized to predict the potential miRNAs which could bind to LARP4. b Venn diagram was used to screen out the target miRNA which could bind to circ_LARP4 and LARP4 simultaneously. RNA pull down assay and qRT-PCR tested the expression of candidate RNAs. c RNA immunoprecipitation (RIP) assay was utilized to verify that circ_LARP4, miR-513b-5p and LARP4 co-existed in RNA-induced-silencing-complex (RISC). d The potential binding sites between circ_LARP4 and miR-513b-5p were depicted by starBase website. e The binding relationship between circ_LARP4 and miR-513b-5p was attested by luciferase reporter assay. f The binding sites between LARP4 and miR-513b-5p were acquired from starBase website. g Luciferase reporter assay was performed to detect the binding correlation between LARP4 and miR-513b-5p. All data were showed as the mean ± SD. **P < 0.01