Fig. 4.

Analysis of the relative expression of proteins involved in the regulation of energy metabolic pathways. Immunoblot analysis of the protein abundance in the CP from Ncbe wt and Ncbe ko mice for a PGC-1α, b TUFM, and c CTCF. d Scatter plot comparing relative changes in protein abundance obtained by proteomic mass spectrometry analysis (MS), and immunoblotting (IB) (*p < 0.05, X: Failed FDR of 1%, n = 5). Mean values are normalized to control (Ncbe wt) and indicated by horizontal bars. Triangles indicate data point from Ncbe wt CP, whereas circles represent data from Ncbe ko CP. e Scatter plot showing the comparative mass spectrometry analysis of additional regulatory proteins Sirtuin-2, Ceacam-2 and general transcription factor II-I between Ncbe wt and Ncbe ko CP (*p < 0.05, X: Failed FDR of 1%, n = 5). Mean values are normalized to control (Ncbe wt) and indicated by horizontal bars. Triangles indicate data points from Ncbe wt CP, whereas circles represent data from Ncbe ko CP