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. 2020 Jan 6;20:23. doi: 10.1186/s12885-019-6408-4

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of NEK1, NEK2, NEK3, and NEK5 in normal and malignant tissues. The expression of a and e) NEK1; b and f) NEK2; c and g) NEK3; d and h) NEK5 was evaluated by immunohistochemistry in normal and malignant tissues of the esophagus, stomach, colon, lung, thyroid, breast, uterine cervix, pancreas, prostate, and kidney. The expression level was calculated through visual (semiquantitative a, b, c, d) and ScanScope score (quantitative e, f, g, h).Two-Way ANOVA and the Bonferroni post-tests correction were employed for statistical analyses: * = P < 0.05; ** = P < 0.01; *** = P < 0.001