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. 2020 Jan 6;20:23. doi: 10.1186/s12885-019-6408-4

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunohistochemistry tissue microarray (TMA) of specimens of human normal and malignant lung and thyroid tissue. The immunostaining of the NEK proteins by using the anti-NEK1 (8 μg/mL), NEK2 (8 μg/mL), NEK3 (8 μg/mL) and NEK5 (8 μg/mL) antibodies. The brownish-red region indicates the immunoreactivity of the advanced biotin-free polymer (DAKO, Carpenteria, CA, USA) with the indicated human NEK protein expressed in the tissue of the lung and thyroid, after being labeled with a chromogenic substrate (DAB - Sigma, St Louis, MA, USA)