Table 1.
Antibodies and Ig controls used for cell surface characterization
| Antibodies/isotype controls | Clone | Fluorochrome | Company | Catalog number |
|---|---|---|---|---|
| CD90 | 5E10 | PERCP Cy5.5 | Biolegend | 328117 |
| CD73 | AD2 | APC | Abcam | ab155378 |
| CD105 | MEM-229 | PE | Abcam | ab53321 |
| CD45 | HI30 | PB | Biolegend | 304021 |
| CD14 | HCD14 | PB | Biolegend | 325615 |
| CD19 | HIB19 | PB | Biolegend | 302223 |
| CD34 | 581 | PB | Biolegend | 343511 |
| CD120a | W15099A | APC | Biolegend | 369906 |
| CD120b | REA520 | PE | Miltenyi Biotec | 130107705 |
| CD119 | GIR-94 | PE | Biolegend | 308703 |
| HLA II | CR3/43 | FITC | Dako | F0817 |
| HLA I | W6/32 | AF 488 | Biolegend | 311413 |
| CD3 | UCHT1 | V450 | BD Bioscience | 560365 |
| CD4 | RPA-T4 | PERCP Cy5.5 | BD Bioscience | 560650 |
| CD25 | M-A251 | PE | BD Bioscience | 555432 |
| CD27 | M-T271 | AF700 | BD Bioscience | 560611 |
| CD127 | HIL-7R-M21 | AF647 | BD Bioscience | 558598 |
| CD45RA | HI100 | PE | Biolegend | 304107 |
| Mouse IgG1 /IgG2a | PE/FITC | BD Bioscience | 342409 | |
| Mouse IgG1, κ | 15H6 | APC | Abcam | ab37391 |
| Mouse IgG1, κ | MOPC-21 | PB | Biolegend | 400131 |
| Mouse IgG1, κ | MOPC-21 | PerCP Cy5.5 | Biolegend | 400149 |
| Mouse IgG1, κ | 11711 | PE | R&D Systems | IC002P |
| Mouse IgG2a | X5563 | PE | Abcam | ab91363 |
APC allophycocyanin, AF Alexa Fluor, PB Pacific Blue, FITC fluorescein isothiocyanate, PE phycoerythrin, PerCP Cy5.5 peridinin chlorophyll protein