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. 2020 Jan 7;11:16. doi: 10.1186/s13287-019-1531-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — IFITM1 regulation in cell migration responses. a Effect of I3C on IFITM1 mRNA expression in TLR4-stimulated hMSCs measured by quantitative real-time PCR. Gene expression was normalized to GAPDH transcript levels. The data represent three independent experiments. **P < 0.005. b Effect of I3C on IFITM1 protein expression in TLR4-stimulated hMSCs measured by western blotting. Protein expression was normalized to β-ACTIN expression level. c, d Quantitative real-time PCR analysis of IFITM1 mRNA in IRF1 siRNA-treated hMSCs and IFITM1 siRNA-treated cells. Gene expression was normalized to GAPDH transcript levels. **P < 0.005. e Effect of knockdown of IRF1 and IFITM1 on the IFITM1 expression in TLR4-stimulated hMSCs assessed by immunofluorescence microscopy. Original magnification, × 400. f The migration of hMSCs was determined using a wound healing migration assay. Representative images of migrating hMSCs after 24 h in TLR4-stimulated or IFITM1 siRNA-treated hMSCs are shown on the left. On the right, the numbers of cells (%) migrated into the middle blank fields after 24 h of incubation are shown. There was a significant difference between the TLR4-stimulated and IFITM1 siRNA-treated hMSCs in the wound healing migration assay when compared with the TLR4-stimulated hMSCs (**P < 0.005)