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. Author manuscript; available in PMC: 2020 Jan 7.
Published in final edited form as: J Vis Exp. 2019 Jan 7;(143):10.3791/58194. doi: 10.3791/58194

Figure 1: c-Fos and Dusp1 are overexpressed in response to growth factor IL-3.

Figure 1:

qPCR analysis of an overexpression of (A) c-Fos and (B) Dusp1 in cytokine-treated BaF3-BA cells. The relative expression was determined after normalization to β-actin. The error bars represent the standard deviation from three replicate samples. (C) Western blot analysis of BaF3-BA cells treated with IL-3 and probed with c-Fos total or P-c-Fos and Dusp1 antibody. Anti-actin antibody was used as loading control. This figure was adapted with permission from Kesarwani et al.13.