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. Author manuscript; available in PMC: 2020 Jan 7.
Published in final edited form as: J Vis Exp. 2019 Jan 7;(143):10.3791/58194. doi: 10.3791/58194

Figure 7: c-Fos and Dusp1 are required for the survival of LSCs.

Figure 7:

(A) Schematic of the transgene used in transgenic mice expressing BCR-ABL1 in stem cells. (B) Experimental design for studying the effects of Dusp1 and c-Fos inhibition by DFC and BCI in vivo. (C) FACS plots of the swing gating strategy used for sorting LSK cells for transplantation. (D) Percentage of the BCR-ABL1 (determined by the percentage of CD45.2 from donor mice) positive cells in mice during and posttreatment with inhibitors. (E) FACS scatter plots showing the CD45 chimerism in live cells, as well as in the LSK cell compartment. Please note the lack of CD45.2 in total and in the LSK compartment in treated mice. This figure was adapted with permission from Kesarwani et al.13.