phasiRNA loci preferentially expressed in soybean meiocytes corresponding with meiocyte-specific transcripts. A, A 21-nt PHAS locus at Glyma.2G099600, which previously was designated as an intergenic PHAS locus. B, A 21-nt PHAS locus corresponds with a meiocyte-specific novel transcript now designated as Glyma.20G001475 (new), which previously was also annotated as an intergenic PHAS locus. C, A 24-nt novel PHAS locus discovered at Chr8: 43,791,331 to 43,792,647 with unconfident transcript. Green arrows in (A) and (B) represent the trigger sites by miR4392. Boxes on top of each screenshot show the phase of each locus. First and second rows of boxes indicate the phasiRNAs from sense and antisense strands, respectively. Numbers indicate the phasing order of phasiRNAs at each locus.