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letter
. 2019 Nov 11;182(1):455–459. doi: 10.1104/pp.19.00859

Figure 1.

Figure 1.

The T0-7 transformant carries a large (>53 kb) deletion spanning across the NRC4 gene cluster. A, Schematic view of the tomato NRC4 gene cluster in wild-type (WT) and mutant T0-7. Orange, NRC4 paralogs; yellow, NRC5; blue, Solyc04g007075, which contains incomplete sequence information due to a sequencing gap in the reference genome. The deleted regions in the mutant T0-7 are marked in gray. B, PCR genotyping for the large deletion. PP1, amplification with primer pair 1:LR1F × 7075F indicated in A; EF1α amplification control with EF1α primers. The uncropped image is provided in Supplemental Figure S2B. C, Sequence alignment and chromatograms of Sanger DNA sequencing results. In this region, the mutant T0-7 contains a 290-bp deletion based on the reference genome and the results of sequencing. D, Sequence alignment and chromatograms of Sanger DNA sequencing results. In this region, the mutant T0-7 contains a 53-kb deletion based on the reference genome and the results of sequencing.