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Detection of cell cycle gene mRNAs in individual meristem cells. A, HIS4 probe was labeled with fluorescein isothiocyanate (FITC) and detected by TSA-FITC (green signals). CYCB1;2 probe was labeled with digoxigenin and detected by TSA-Cy5 (red signals). HIS4 and CYCB1;2 mRNAs were observed in different cells, indicating a nonoverlapping expression pattern during the cell cycle. B, Enlargement of the region outlined in A. The nucleus (blue) was labeled by DAPI staining. Bars = 25 μm.
