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. 2019 Nov 13;182(1):147–158. doi: 10.1104/pp.19.00980

Figure 5.

Figure 5.

Subcellular localization of cell cycle mRNAs in the cytoplasm and the nucleus. CYCB1;1 and CDC20 probes were labeled with fluorescein isothiocyanate (FITC) and detected by TSA-FITC (green signals). MAD2 probe was labeled with digoxigenin and detected by TSA-Cy5 (red signals). A, Colocalization of CYCB1;1 and MAD2 mRNAs in the cytoplasm of mitotic cells. The arrowhead indicates a prophase cell that expresses CYCB1;1 and MAD2. B, Nucleocytoplasmic separation of CDC20 and MAD2 mRNAs in the same cells. Arrows indicate prophase cells that coexpress CDC20 and MAD2. CDC20 mRNAs are retained in the nucleus, whereas MAD2 mRNAs are exported into the cytoplasm. The bottom row shows enlargement of the regions outlined in the top row. Bars in top rows = 25 μm and bars in bottom rows = 10 μm.