Figure 2.

N-responsive reprogramming of gene expression is altered in the sdg8-5 deletion mutant. Expression levels of 215 genes significantly regulated by the interaction of nitrate and SDG8 genotype are shown as a heatmap. Two major clusters of the 215 DEGs were identified: Cluster 1 comprises 126 genes that are more highly induced by N starvation in wild type (WT), compared to the sdg8-5 mutant; the cluster-1 genes share a significant overlap with the genes that display higher H3K36me3 under the N-starved condition than the N-supplied condition. Cluster 2 contains 89 genes induced by N supply in wild type, which is abolished or attenuated in the sdg8-5 mutant; the cluster-2 genes share a significant overlap with genes that exhibit higher H3K36me3 under the N-supplied condition than the N-starved condition.