Figure 3.

Acclimation to 530 µmol photons m⁻² s⁻¹ of red light followed by recovery in CC-4533 (wild type) and Crvtc2-1 cultures grown photomixotrophically in TAP medium at 100 µmol photons m⁻² s⁻¹. A, NPQ kinetics. Data were analyzed by one-way ANOVA followed by Dunnett’s posttest: ##, P < 0.01, compared to the CC-4533 strain at the respective time point. µE, µmol photons m⁻² s⁻¹. B, De-epoxidation index. C, The F_V/F_M value, an indicator of photosynthetic efficiency, parameter measured after dark adaptation and after recovery from the 530 µmol photons m⁻² s⁻¹ red light. D, The 684/710 nm ratio of the 77 K fluorescence spectra. Samples were collected at the growth light of 100 µmol photons m⁻² s⁻¹ after 30 min of dark adaptation, at the end of the 30-min light period with 530 µmol photons m⁻² s⁻¹, and 15 min after the cessation of actinic illumination, as indicated by arrows in the scheme in A. The presented data are based on five independent experiments. When applicable, averages and ses (±se) were calculated. Data were analyzed by one-way ANOVA followed by Dunnett’s posttest: ×, P < 0.05, ××, P < 0.01, and ×××, P < 0.001, compared to the dark-adapted CC-4533 strain.