Figure 4.

Effects of overnight (16 h) dark acclimation on CC-4533 and Crvtc2-1 grown in TAP medium at 100 µmol photons m⁻² s⁻¹. A, Ascorbate content after 16 h of dark acclimation. N.D., not detectable. B, NPQ, induced by 530 µmol photons m⁻² s⁻¹ of red light after overnight dark acclimation. µE, µmol photons m⁻² s⁻¹. C, De-epoxidation index, determined in the overnight dark-acclimated cultures following red-light illumination and recovery. The presented data are based on four independent experiments. When applicable, averages and ses (±se) were calculated. Data were analyzed by one-way ANOVA followed by Dunnett’s posttest: ×××, P < 0.001, and ××××, P < 0.0001, compared to the dark-acclimated CC-4533 strain.