Figure 7.

EIN3 is associated with the promoter regions of SID1, SID2, and ICS2. A, Schematic diagram of DNA fragments used for ChIP experiments and the EIN3-binding motifs found in the promoters of SID1, SID2, EDS1, and ICS2. Triangles indicate the positions of the EIN3-binding motifs relative to the translation start site. B and C, ChIP assays of EIN3 binding to the promoter regions of SID1 (B) and ICS2 (C). Shown are the qPCR results from samples precipitated with antibody (Ab) or without antibody (nAb) of GFP. SID2 was used as a positive control. Significant differences between samples were determined by Student’s t test; ns, no significant difference. Values represent means ± sd from two independent experiments.