Transcription termination activity of MBP:mTERF8 in vitro. A, Transcription termination activity of MBP:mTERF8 in vitro. MBP:mTERF8 was added to the T7 RNA polymerase transcription system in vitro with the Arabidopsis psbJ gene as a template. B to F, Transcription termination activity of MBP:mTERF8 in vitro. MBP:mTERF8 was added to the T7 RNA polymerase transcription system in vitro with the Arabidopsis psbA, rbcL, psaJ, petD, and petA genes as a template. All the above transcripts were separated by electrophoresis on a 2% agarose gel, then stained with ethidium bromide.