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. Author manuscript; available in PMC: 2020 Dec 26.
Published in final edited form as: J Am Chem Soc. 2019 Dec 16;141(51):20166–20170. doi: 10.1021/jacs.9b09080

Figure 2. Translation of unnatural codons in HEK293T cells.

Figure 2.

A. Average (± s.d.; n = 3) EGFP fluorescence signal (total fluorescence signal divided by the number of EGFP-positive cells) of HEK293T cells transfected with unnatural codons with or without cognate tRNAs measured by flow cytometry. *P < 0.05, **P < 0.005, ***P < 0.0005 (two-tailed paired t test). B. The protein shift assay for HEK293T cells transfected with unnatural codon GXC using cell lysate. Representative gels from 3 biological replicates.