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. Author manuscript; available in PMC: 2020 Dec 26.
Published in final edited form as: J Am Chem Soc. 2019 Dec 16;141(51):20166–20170. doi: 10.1021/jacs.9b09080

Figure 3. Translation of unnatural codons in CHO cells.

Figure 3.

A. Average EGFP fluorescence signal (total fluorescence signal divided by the number of EGFP-positive cells) of CHO cells transfected with unnatural codons with or without cognate tRNAs (and self-pairing tRNA for codon AGX) measured by flow cytometry. *P < 0.05, **P < 0.005, ***P < 0.0005 (two-tailed paired t test). B. The protein shift assay for CHO cells transfected with indicated components. Representative gels from 3 biological replicates.