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. 2020 Jan 7;15(1):e0227030. doi: 10.1371/journal.pone.0227030

Fig 5. EVs obtained from U937 cells increased the expression of adhesion molecules in endothelial cells EA.hy926.

Fig 5

EA.hy926 cells seeded on transwell® inserts were exposed to the different evaluated conditions for 24 h p.e., then the cells were processed by immunofluorescence (IF) to detect the proteins VE-Cad, E-Selectin (E-Sele) or ICAM. Cells infected with DENV-2 at MOI 0.2 (IC) showed a slight decrease in the expression of VE-Cad, while in cells exposed to P (+ or -) or EV (+ 4G2 or UV) showed a bright fluorescence pattern that delimits the cells. E-Selectin (E-sele) was detected in the cytoplasm of IC and slightly in endothelial cells exposed to EVs (+) 4G2 or UV. Similarly, in the infected cells ICAM was detected in the cytoplasm, while in cells exposed to P (+) or EVs (+) 4G2 the labeling was observed on the cell surface. Photomicrographs are representative of two duplicate experiments. Bar: 10 and 50 μm.